The bacterial immune system “Clustered Regularly Interspaced Short Palindromic Repeats” CRISPR revolutionized genome engineering by enabling democratization of genome editing to a broad range of organisms including some resilient to genetic modification. The workshop will tackle topics beyond the unprecedently accurate gene editing with the Streptococcus pyogenes Cas9, by also discussing emergence of new CRISPR endonucleases, off-targeting and impact on the synthetic biology field. Where: Kronigzaal, Department of Biotechnology (Building 58), Delft University of Technology, van der Maasweg 9, 2629HZ Delft.
Attendance to the workshop is free of charge but the number of seats is limited to 50.
Schedule of Application of CRISPR technologies in microbial biotechnology
| On Thursday 21 February: | |
| 12:30 - 13:30 | Lunch |
| 13:30 - 13:45 | Jean-Marc Daran (TUD) - Biotechnology |
| 13:45 - 14:15 | Prof. John van der Oost (WUR) - New/other CRISPR enzymes |
| 14:15 - 14:45 | To be announced |
| 14:45 - 15:15 | Arthur Gorter de Vries (TUD) - Allele-specific genome editing using CRISPR-Cas9 is associated with loss of heterozygosity in diploid |
| 15:15 - 15:45 | Break |
| 15:45 - 16:15 | Dr Martin Depken (Bionanosciences TUD) - Understanding CRISPR-Cas off-targeting through kinetic modelling |
| 16:15 - 16:45 | Pascale Daran Lapujade (Biotechnology TUD) - Synthetic chromosome in S. cerevisiae |
| 16:45 - 17:15 | Christophe Danelon (Bionanosciences TUD) - Synthesizing a cell |
| 17:15 - 17:45 | Discussion and closing |
| 17:45 - 18:30 | Drinks |
