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Over Application of CRISPR technologies in microbial biotechnology

Application of CRISPR technologies in microbial biotechnology

The bacterial immune system “Clustered Regularly Interspaced Short Palindromic Repeats” CRISPR revolutionized genome engineering by enabling democratization of genome editing to a broad range of organisms including some resilient to genetic modification. The workshop will tackle topics beyond the unprecedently accurate gene editing with the Streptococcus pyogenes Cas9, by also discussing emergence of new CRISPR endonucleases, off-targeting and impact on the synthetic biology field. Where: Kronigzaal, Department of Biotechnology (Building 58), Delft University of Technology, van der Maasweg 9, 2629HZ Delft.

Attendance to the workshop is free of charge but the number of seats is limited to 50.

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Programma van Application of CRISPR technologies in microbial biotechnology

Op donderdag 21 februari:
12:30 - 13:30 Lunch
13:30 - 13:45 Jean-Marc Daran (TUD) - Biotechnology
13:45 - 14:15 Prof. John van der Oost (WUR) - New/other CRISPR enzymes
14:15 - 14:45 To be announced
14:45 - 15:15 Arthur Gorter de Vries (TUD) - Allele-specific genome editing using CRISPR-Cas9 is associated with loss of heterozygosity in diploid
15:15 - 15:45 Break
15:45 - 16:15 Dr Martin Depken (Bionanosciences TUD) - Understanding CRISPR-Cas off-targeting through kinetic modelling
16:15 - 16:45 Pascale Daran Lapujade (Biotechnology TUD) - Synthetic chromosome in S. cerevisiae
16:45 - 17:15 Christophe Danelon (Bionanosciences TUD) - Synthesizing a cell
17:15 - 17:45 Discussion and closing
17:45 - 18:30 Drinks

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